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         AAAS/Science presents "Getting the best data from your cells: From tissue culture to final analysis."


Speakers:

Christopher Korch, Ph.D.

University of Colorado Anschutz Medical Campus, Aurora, CO

Karina Eterovic, Ph.D.

The University of Texas MD Anderson Cancer Center, Houston, TX

Richard M. Neve, Ph.D.

Gilead Sciences Inc., Foster City, CA 


To view the webinar, please
copy and paste this URL into a new browser window:  http://www.sciencemag.org/custom-publishing/webinars/getting-best-data-your-cells-tissue-culture-final-analysis  

            

 

 

 

The New SDO Newsletter is now Available!!

 

ANSI standard ASN-0002 Authentication of Human Cell Lines: Standardization of STR Profiling to be reviewed, revised, and reaffirmed as  per the continuous maintenance. 

 

 

The ATCC Standards Development Organization (SDO) has a new standard in development.......ASN-0004 Species-Level Identification and Cross-Contamination Screening in Animal Cells by Multiplex PCR.  Work group members are fine tuning  and finalize the optimal conditions for the assay now. A draft will be made available for public review in the near future!

 

 

I am very pleased to announce that ANSI/ATCC-0003, Species-Level Identification of Animal Cells through Mitochondrial Cytochrome c Oxidase Subunit 1 (CO1) DNA Barcodes is now available through the ANSI estores: http://webstore.ansi.org/RecordDetail.aspx?sku=ANSI%2fATCC+ASN-0003-2015

 

 

 

 

For program details, and to register  go to  http://www.isbiotech.org/

 

 

 

   iclac.org/cancer-moonshot/

 

 

Solving Identity Crisis in Animal Cells; Best Practices with DNA Barcodes and STR Analysis

Recorded March 31, 2016

Speakers:
Yvonne Reid, Ph.D., Manager, Scientist, ATCC
Jason Cooper, M.S., Adjunct Professor, Community College of Allegheny County

Abstract: Animal cell lines are important in vitro systems and tools for scientists in diverse disciplines such as basic cell biology, genetic mapping, gene expression, and gene therapy. Animal cell line identity, which is a crucial first step in cell line authentication, is frequently underappreciated and ignored by most research scientists. Over the years numerous cell lines have been shown to be misidentified due, in part, to poor techniques and inadequate authentication protocols. It is estimated that the financial loss incurred by misidentified cell lines is in the millions of dollars. Animal cell line identity now requires a comprehensive strategy that employs several complementary technologies. Technological advances have given rise to improved capabilities. An overview of these technologies to include STR analysis for human cell line identity and CO1 for animal cell line identity will be presented.

Key points:

·        Cell line authentication is critical to meet funding, quality control, and publication requirements

·        Short tandem repeat (STR) profiling is an accepted form of authentication that differentiates human cell lines

·        DNA barcoding complements STR profiling by providing species-specific identification 

Biographies: Yvonne A. Reid, Ph.D., is a Manager, Scientist at ATCC. Dr. Reid has been at the forefront of developing and standardizing methods to characterize cell lines by enzymology, karyotyping, and more recently DNA profiling, and to propagate, cryopreserve, and track validated batches of thousands of animal cell lines and strains. These validation protocols have been adopted by cell banks around the world. In addition, Dr. Reid has been heavily involved in developing guidelines for authenticating cell lines and cell types.

Jason Cooper, M.S., is a Biology Professor (Adjunct) at the Community College of Allegheny County in Pittsburgh, PA and a Science Writer at Thermo Fisher Scientific. He has worked as a scientist, researcher, and supervisor at Roche Molecular Systems, Aptagen, Inc., as well as ATCC. Most recently, Mr. Cooper has been pivotal in the development and adoption of the consensus standard ASN-0003 for interspecies cell line identity, based on DNA barcoding. He is currently working on a new standard for species level identification and cross-contamination screening in animal cells by multiplex PCR.

 

     

Ø   Come join us for the ATCC Learning Center webinars!

            All Webinars are open to the public

 

Ø ATCC SDO proudly announces its second standard, ASN-0002: Authentication of Human Cell Lines: Standardization of STR Profiling. Read how this New Standard Will Help Correct Cell Line Misidentification. ASN-0002 is now available on the ANSI e stores.

  

Ø SOCIAL MEDIAThe ATCC SDO actively participates in two social media websites, making pertinent information about events and initiatives readily accessible and providing a place of discussion for the SDO membership.Check us out on LinkedIn and Facebook 
    
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